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Pureline Biolabs Ltd · Company No. 17236739 · Registered in England & Wales · Not evaluated by the MHRA
A laboratory dilution calculator. Enter the mass of lyophilised peptide and the volume of solvent added, and it returns the resulting concentration in mg/ml and the volume of solution containing a given mass. Results are mathematical conversions for preparing laboratory solutions. They are not dosing advice, no administration values are provided, and the calculator does not establish sterility, stability or fitness for any particular application.
Results are mathematical conversions for preparing laboratory solutions, not dosing advice. No human or veterinary administration values are provided. Users must select methods suitable for their own validated experiment, and verify all calculations independently. This calculator does not establish sterility, stability or fitness for any particular application. Pureline Biolabs materials are supplied for laboratory research purposes only — not for human or veterinary use.
The calculator works out laboratory solution concentrations from the vial quantity and diluent volume entered. For information about solvent selection, handling, dissolution and storage, read our complete guide to reconstituting lyophilised research peptides.
You can also review the specification for bacteriostatic water for laboratory reconstitution.
A two-page A4 PDF for the bench. Page one is a concentration reference chart covering every vial size against 1ml, 2ml, 3ml and 5ml of diluent, plus the draw-volume formula and the solubility rules by net charge. Page two is a printable reconstitution and storage log: date, compound, batch, diluent, volume, concentration, storage and initials. Print it, pin it up, keep it with your batch certificates.
Download the PDF A4 · 2 pages · free to print, share and use in your own laboratoryThere is no hidden arithmetic. Concentration is the peptide mass in the vial divided by the volume of bacteriostatic water added, and the volume to draw is the mass you want divided by that concentration. Both are shown below so the result can be checked by hand.
Concentration (mg/ml) = peptide mass (mg) ÷ diluent volume (ml)
Volume to draw (ml) = mass wanted (mg) ÷ concentration (mg/ml)
Worked through: a 10mg vial with 2ml of bacteriostatic water gives 5mg/ml. To obtain 1mg from that solution you draw 1 ÷ 5 = 0.200ml. Halve the diluent to 1ml and the same vial gives 10mg/ml, so the same 1mg now sits in 0.100ml.
One caveat the arithmetic cannot capture: the mass printed on a vial is gross weight, which includes counter-ion salt and residual water. Actual peptide content is lower. Our guide to what peptide purity figures actually mean explains the difference between HPLC area-percent purity and net peptide content.
Resulting concentration in mg/ml for each vial size against common diluent volumes. The same figures are on page one of the printable chart above.
There is no single correct volume. More diluent gives a weaker solution and larger draw volumes; less gives a stronger solution and smaller ones. Two millilitres is a common working choice because it produces convenient draws on a standard insulin syringe. Figures below assume 2ml.
2ml gives 25mg/ml. GHK-Cu is the one compound where a pale blue solution is correct rather than a fault, because it is a copper complex. Clear blue is expected; cloudy is not.
2ml gives 5mg/ml for the 10mg vial and 10mg/ml for the 20mg vial. Check which size you are holding before calculating, because the two are indistinguishable once in solution.
2ml gives 2.5mg/ml. Because the vial mass is lower, the same diluent volume produces a weaker solution and correspondingly larger draw volumes than a 10mg vial.
2ml gives 5mg/ml. The finished solution should be clear and colourless.
2ml gives 5mg/ml. Kisspeptin-10 degrades faster than most peptides in solution, so reconstitute the smallest practical volume and use it sooner rather than later.
All are 10mg vials, so 2ml gives 5mg/ml in each case. Semax is also supplied as a 5mg vial, which gives 2.5mg/ml at the same volume. Select the exact size in the calculator above and it will return the batch certificate alongside the result.
Is the peptide reconstitution calculator free?
Yes, and there is no email wall. The calculator is free to use and the printable chart above is free to download, print and share. You do not need an account and you do not need to buy anything.
Can I use it for peptides I did not buy from Pureline?
Yes. The arithmetic is the same whoever supplied the vial. Selecting a Pureline product simply pre-fills the vial mass and pulls in that batch certificate; you can enter any mass and volume manually instead.
What does mg/ml mean?
Milligrams of peptide per millilitre of solution. It is the concentration, and it is what determines how much volume contains a given mass. A 10mg vial in 2ml is 5mg/ml, so 1mg sits in 0.200ml.
How accurate is the result?
The arithmetic is exact. What it cannot know is the true peptide content of your vial, because a labelled mass is gross weight including counter-ion salt and residual water. Treat the output as the concentration implied by the label, not a measured assay value.
Why does it show a batch certificate?
Because a concentration is only as meaningful as the material it describes. Selecting a Pureline compound returns the batch number, the independently measured purity and a link that verifies on Janoshik Analytical's own portal rather than on this site.
